Product Name: Human RYR3 Primer Pair 1
Product Type : RT-PCR Primers
Code : CSB-PP02062111h
Size : 25T
Abbreviation : RYR3
Storage : The Primer Pair is stable for up to one year at -20° C in a non-frost free freezer. Avoid repeated freeze-thaw cycles.
Relevance : Human RYR3 Primer Pair 1 for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.
Tested Applications : RT-PCR
Accession NO. : NM_001036
Length/Coupling Ratio : 992bp

Quality Control : Primer Pairs for RT-PCR provide convenient primers for analyzing the expression of specific mRNAs or got specific PCR fragment by RT-PCR. Each Primer Pair includes a 5′ and 3′ oligonucleotide primer provided in individual vials at 50μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR using human pancreatic carcinoma SW1990 cells or human lung carcinoma A549 epithelial cells total RNA. PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.
Technical Hints : ? Thaw all reagents completely on ice before use. ? Use either random primers or oligo (dT) for reverse transcription. ? All PCR reactions should be assembled on ice. ? The recommended annealing