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Product Type :
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ELISA kit
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Code :
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CSB-EQ028056HU
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Size :
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96T,5×96T,10×96T
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Abbreviation :
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MR-proANP
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Species :
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Human
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Sample Types :
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serum, plasma, tissue homogenates
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Detect Range :
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39 pmol/L-2500 pmol/L
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Sensitivity :
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9.8 pmol/L.
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Assay Time :
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1-3h
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Sample Volume :
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50-100ul
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Detection Wavelengt :
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450nm
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Research Area :
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Others
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Protocol :
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Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
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Precision :
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Intra-assay Precision (Precision within an assay): CV%<8%
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Three samples of known concentration were tested twenty times on one plate to assess.
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Inter-assay Precision (Precision between assays): CV%<10%
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Three samples of known concentration were tested in twenty assays to assess.
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Linearity :
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To assess the linearity of the assay, samples were spiked with high concentrations of human MR-proANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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Sample
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Serum(n=4)
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1:1
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Average %
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94
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Range %
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85-97
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1:2
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Average %
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100
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Range %
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94-105
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1:4
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Average %
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90
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Range %
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83-94
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1:8
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Average %
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97
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Range %
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91-101
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Recovery :
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The recovery of human MR-proANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
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Sample Type
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Average % Recovery
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Range
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Serum (n=5)
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95
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89-98
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EDTA plasma (n=4)
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102
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92-107
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