Mouse Monoclonal (IgG1) 1mg/ml in PBS, pH 7.4 with 0.05% sodium azide. Frozen liquid.Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows:  Western blot at dilution of 1:1000-4000 Immunofluorescence staining at 1:100-400 dilutions. It is very useful as a loading control for quantitative western blotting. The antibody recognizes a 50kDa band in a Western Blot analysis.