INTENDED USE

 

The One-Step Fecal Occult Blood (FOB) Diagnostic Kit is a qualitative detection of human occult blood in feces for self-testing.

 

PRINCIPLE

 

Immune Colloidal Gold Method: The One-Step Fecal Occult Blood (FOB) Diagnostic Kit is an immunochromatographic sandwich method, which employ two specific monoclonal antibodies to selectively identify hemoglobin in test samples. The result is very specific, and easier to interpret than those of guaiac-based test. The sensitivity is very high with the ability to detect 200ng/ml hemoglobin in feces. In addition, the accuracy of the test is not affected by interfering substances, and dietary restriction is not necessary.

Chemical Method: Hemoglobin has peroxidase activity which can cause peroxide decompose then release oxygen atom. The oxygen atom oxidizes 3,3', 5, 5'-tetramethyl-benzidine indicator to change the color. When the sample has human hemoglobin, the resulting colors range from orange to yellow-green or dark green. Very high blood concentration may cause the color development to continue to dark blue.

 

REAGENTS AND MATERIALS PROVIDED

 

1.    Test Device: A pouch contains a single test for FOB and a desiccant. 25 pouches in a box.

2.    Sample collection (with diluent)    

3.    Operating Instruction