Product Description

Reliable quality and low price. Low to 10$/10000 Units( 50 applications)

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant:
Description: Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus, Point Mutant (M-MLV RT (H minus) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is germane to this application in that this activity can start to degrade template when incubation times are long, as they may need to be when making long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option for the preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.
An application of M-MLV RT (H minus) is first-strand synthesis of cDNA from RNA molecules.

Enzyme Storage Buffer: M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant is supplied in 20mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.
M-MLV Reverse Transcriptase 5X Reaction Buffer: When the M-MLV Reverse Transcriptase 5X Reaction
Buffer supplied with this enzyme is diluted 1: 5, it has a composition of 50mM Tris-HCl (pH 8.3), 75mM KCl, 3mM MgCl2 and 10mM DTT.
Source: Purified from an E. Coli strain expressing a recombinant enzyme
Storage Temperature: Store at minus 20.