The Eco-Friendly PCR Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures. The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. The PCR Purification Kit effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. Each purification column has a total binding capacity of up to 30μg of DNA and the entire procedure takes just 15 min. The purified DNA can be used in common downstream applications such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.