The TP Rapid Screen Test is a qualitative test for the detection of antibodies to Treponema pallidum in human serum or plasma. It is considered as an initial screening test for TP antibodies. All positive specimens must be confirmed with Western Blot or other qualified EIA.

 

PRINCIPLE:

1.

specimen is allowed to react with the colloidal gold particles, which have been labeled with TP specific antigens. Antibodies to TP, if present, a purple colored band will develop on the membrane in proportion to the amount of TP antibodies present in the specimen.

2.

Absence of this purple colored band in the test region suggests a negative result. To serve as a procedural control, a purple colored band in the control region will always appear regardless the presence of antibodies to TP.

 

PROCEDURE:

Serum or Plasma Sample

Add 70-100 ul or 2-3 drops of serum or plasma into sample well. Observe the result in 10-15 minutes.

 

Whole Blood Sample

Add 1 drop of whole blood into sample well, after all blood completely absorbed. Add 1 drop of whole blood diluent. Observe the result in 10-15 minutes