Purpose

This kit allows for the determination of CYP21B concentrations in Human serum, blood plasma, and other biological fluids.

 

Principle of the assay

The kit assay Human CYP21B level in the sample, use Purified Human CYP21B antibody to coat microtiter plate wells, make solid-phase antibody, then add CYP21B to wells, Combined CYP21B antibody which With HRP labeled , become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CYP21B in the samples is then determined by comparing the O.D. of the samples to the standard curve.