Intended Use

This kit is used  to assay the Endothelin-1(ET-1) in the sample of human’s serum, blood plasma, and other related tissue Liquid.

 

Test principle

The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Endothelin-1(ET-1) in samples. Add Endothelin-1(ET-1) to monoclonal antibody Enzyme well which is pre-coated with Human Endothelin-1(ET-1)monoclonal antibody, incubation; then, add Endothelin-1(ET-1)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concenthumanion of the Human Substance Endothelin-1(ET-1) of sample were positively correlated.