Intended Use
This kit is used to assay the Free cholesterol(FC) in the sample of human’s serum, blood plasma, and other related tissue Liquid.
Test principle
The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Free cholesterol(FC) in samples. Add Free cholesterol(FC) to monoclonal antibody Enzyme well which is pre-coated with Human Free cholesterol(FC)monoclonal antibody, incubation; then, add Free cholesterol(FC)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concenthumanion of the Human Substance Free cholesterol(FC) of sample were positively correlated.
Materials supplied in the Test Kit
|
1
|
Standard(960nmol/L)
|
0.5ml
|
7
|
Chromogen Solution A
|
6ml
|
|
2
|
Standard diluent
|
3ml
|
8
|
Chromogen Solution B
|
6ml
|
|
3
|
Microelisa Stripplate
|
12well×8strips
|
9
|
Stop Solution
|
6ml
|
|
4
|
Str- HRP-Conjugate Reagent
|
6ml
|
10
|
Instruction
|
1
|
|
5
|
30×wash solution
|
20ml
|
11
|
Closure plate membrane
|
2
|
|
6
|
Biotin-FC Ab
|
1ml
|
12
|
Sealed bags
|
1
|
Materials required but not supplied
1.37 ℃ incubator
2.Standard Enzyme reader
3.Precision pipettes and Disposable pipette tips
4.Distilled water
5.Disposable tubes for sample dilution
6.Absorbent paper