Purpose
This kit allows for the determination of IGF-1 concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human IGF-1 level in the sample, use Purified Human IGF-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add IGF-1 to wells, Combined antibody which With HRP labeled goat anti-Human become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at wavelength of 450 nm. The concentration of IGF-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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Materials provided with
the kit
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48 determinations
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96 determinations
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Storage
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User manual
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1
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1
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Closure plate membrane
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2
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2
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Sealed bags
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1
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1
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Microelisa stripplate
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1
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1
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2-8℃
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Standard: 270μg/L
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0.5ml×1 bottle
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0.5ml×1 bottle
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2-8℃
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Standard diluent
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1.5ml×1 bottle
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1.5ml×1 bottle
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2-8℃
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HRP-Conjugate reage
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Sample diluent
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Chromogen Solution A
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Chromogen Solution B
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Stop Solution
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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wash solution
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(20ml×20 fold)
×1bottle
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(20ml×30 fold)
×1bottle
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2-8℃
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