Purpose

This kit allows for the determination of IL-31 concentrations in Human serum, blood plasma, and other biological fluids.

 

Principle of the assay

The kit assay Human IL-31 level in the sample, use Purified Human IL-31antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-31 to wells, Combined IL-31 antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-31 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Materials provided with the kit