Intended Use

The HAV test is a rapid qualitative lateral flow test designed for the qualitative detection of hepatitis A virus infection (HAV) in serum/plasma.

Principle of the Test

The quick one-step test utilizes a sandwich immunochromatographic detection assay platform, If HAV antibody IgM is present in the sample in concentration above the limit, a labeled antigen-dye complex will be formed. This complex is then captured by antibody immobilized in the Test Zone of the membrane, producing a visible pink color band on the membrane. The intensity of color will depend on the concentration of HAV antibody IgM present in the sample.

Materials Provided 

Test devices ,    Disposable specimen pipette    

SPECIMEN COLLECTION

For serum(or plasma), collect blood into a container without anticoagulant. If the specimen cannot be tested on the day of collection, store the serum (or plasma) specimen in a refrigerator or freezer. Bring the specimens to room temperature before testing.

PROCEDURE

1.         Open a pouch containing a cassette, lay the cassette.

2.         Carefully apply 10ul of sample onto 10ml buffer solution(1:1000),  then drop 75ul(3 drops)well mixed buffer onto the sample well.

3.         Read results within 10 minutes.