Product Description
Procedure Overview
Specificity
REAGEN™Fumonisin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Fumonisin in cereals, dried meat/fish, feed, milk and seed .
The unique features of the kit are:
§ High recovery (>80%),rapid(10-20minutes), cost-effective extraction methods.
§ High sensitivity (1 ng/g or ppb).
§ A quick ELISA assay (less than 1 hours regardless of number of samples).
§ High reproducibility.
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Fumonisin ELISA Test Kit has the Fumonisinacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
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Kit Contents
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Amount
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Storage
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Fumonisin Antibody-Coated Plate
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1 x 96-well Plate (8 wells x 12 strips)
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2-8°C
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Fumonisin Standards:
Negative control (white cap tube)
1 ng/mL (yellow cap tube)
5 ng/mL (orange cap tube)
10 ng/mL (pink cap tube)
25ng/mL (purple cap tube)
50 ng/mL (blue cap tube)
1000 ng/mL (spiking, red cap tube)
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0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
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2-8°C
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Fumonisin Antibody #1
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8ml
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2-8°C *
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100X HRP-Conjugated Antibody #2
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120 mL
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2-8°C
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Antibody #2 Diluent
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20 mL
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2-8°C
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20X Wash Solution **
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28 mL
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2-8°C
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Stop Buffer **
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20 mL
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2-8°C
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TMB Substrate **
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12 mL
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2-8°C
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* If you are not planning to use the kit for over 3 months, store Fumonisin Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C or in a freezer.
Sensitivity
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Sample Type
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Detection Limit (ng/g or ppb)
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Meat/fish/sereal/feed/seed
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10
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Milk
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10
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Analytes
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Cross-Reactivity (%)
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Fumonisin B1
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100.0
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Fumonisin B2
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94
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Fumonisin B3
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145
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T-2
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<0.1
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