Product Description
Procedure Overview
Kit Contents, Storage and Shelf Life
Sensitivity
Specificity
REAGEN™Total Aflatoxin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Total Aflatoxin in cereals, meat/fish, feed, milk ,oils,peanuts and pistachios .
The unique features of the kit are:
§ High recovery (>80%),rapid(10-40minutes), cost-effective extraction methods.
§ High sensitivity (0.05 ng/g or ppb).
§ A quick ELISA assay (less than 2 hours regardless of number of samples).
§ High reproducibility.
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
REAGEN™Total Aflatoxin ELISA Test Kit has the Total Aflatoxinacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
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Kit Contents
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Amount
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Storage
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Aflatoxin Antibody-Coated Plate
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96-well Plate (8 wells x 12 strips)
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2-8°C
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Aflatoxin Standards:
Negative control (white cap tube)
0.05ng/mL (yellow cap tube)
0.1ng/mL (orange cap tube)
0.2 ng/mL (pink cap tube)
0.4ng/mL (purple cap tube)
0.8 ng/mL (blue cap tube)
100 ng/mL (spiking, red cap tube)
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0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
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2-8°C
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Total Aflatoxin Antibody #1
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8ml
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2-8°C*
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HRP-Conjugated Antibody #2
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18 mL
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2-8°C
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20X Wash Solution **
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28 mL
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2-8°C
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Stop Buffer **
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20 mL
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2-8°C
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TMB Substrate **
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12 mL
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2-8°C
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* If you are not planning to use the kit for over 3 months, storel Aflatoxin B 1 Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C or in a freezer.
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Sample Type
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Detection Limit (ng/g or ppb)
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Meat/fish/sereal/feed/seed/starch
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0.5
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Meat/liver/kidney
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0.5
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Milk
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0.5
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Milk powder
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4.5
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Peanut butte
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0.5
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Analytes
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Cross-Reactivity (%)
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Aflatoxin B1
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100.0
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Aflatoxin B2
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76
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Aflatoxin M1
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79
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Aflatoxin M2
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33
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Aflatoxin G1
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55
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Aflatoxin G2
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6
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