Product Description
REAGEN™DON ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of DON in wheat,barley, oats, rye, and maize.
The unique features of the kit are:
§ High recovery (75% - 95%), and cost-effective extraction methods .
§ A quick ELISA assay (less than 1.5 hours regardless of number of samples).
§ High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The DON antibody has been coated in the plate wells. During the analysis, sample is added along with the DON-horseradish peroxidase (DON-HRP) conjugate. If the DON residue is present in the sample, it will compete for the DON antibody, thereby preventing the DON-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the DON residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™DON ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
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Kit Contents
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Amount
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Storage
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DON Antibody-Coated Plate
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1 x 96-well plate (8 wells x 12 strips)
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2-8°C
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DON Standards:
Negative control (white cap tube)
2.5 ng/mL (yellow cap tube)
5ng/mL (orange cap tube)
10 ng/mL (pink cap tube)
25 ng/mL (purple cap tube)
50 ng/mL (blue cap tube)
1000 ng/mL (spiking, red cap tube)
|
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
|
2-8°C
|
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DON- HRP Conjugate
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8 mL
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2-8°C *
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10X Sample Extraction Buffer **
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25 mL
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2-8°C
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20X Wash Solution **
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28 mL
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2-8°C
|
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Stop Buffer **
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20 mL
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2-8°C
|
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TMB Substrate **
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12 mL
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2-8°C
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* If you are not planning to use the kit for over 1 month, store DON- HRP Conjugate at -20°C or in a freezer.
Sensitivity
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Sample Type
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Detection Limit (ng/g or ppb)
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Cereals/Feed(low concentration)
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15
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Cereals/Feed(high concentration)
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1000
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Specificity
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Analytes
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Cross-Reactivity (%)
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DON
|
100
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3-AC-DON
|
452
|
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15-AC-DON
|
4.2
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Triacetyl-DON
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0.5
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T-2 toxin
|
<0.1
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T-2 triol
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<0.1
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Nivalenol
|
<0.1
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