Product Description
Procedure Overview
Kit Contents, Storage and Shelf Life
REAGEN™ Diethylstilbestrol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Diethylstilbestrol in meat, fish, feed and urine samples.
The unique features of the kit are:
§ High recovery (75-95%),rapid(10-30minutes), cost-effective extraction methods.
§ High sensitivity(0.15 ng/g or ppb) and low detection limit (meat/fish0.075 ppb and urine 0.15ppb).
§ A quick ELISA assay (less than 2 hours regardless of number of samples).
§ High reproducibility.
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
REAGEN™ Diethylstilbestrol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
|
Kit Contents
|
Amount
|
Storage
|
|
Diethylstilbestrol Coated Plate
|
1 x 96-well Plate (8 wells x 12 strips)
|
2-8°C
|
|
Diethylstilbestrol Standards:
Negative control (white cap tube)
0.15ng/mL (yellow cap tube)
0.5ng/mL (orange cap tube)
1.5 ng/mL (pink cap tube)
4.5ng/mL (purple cap tube)
9.0 ng/mL (blue cap tube)
20 ng/mL (spiking, red cap tube)
|
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
|
2-8°C
|
|
Diethylstilbestrol Antibody #1
|
15mL
|
2-8°C *
|
|
100X HRP-Conjugated Antibody #2
|
250 mL
|
2-8°C
|
|
HRP Conjugate Antibody #2 Diluent
|
20 mL
|
2-8°C
|
|
20X Wash Solution **
|
28 mL
|
2-8°C
|
|
Stop Buffer **
|
20 mL
|
2-8°C
|
|
TMB Substrate **
|
12 mL
|
2-8°C
|
|
10X PBS
|
25mL
|
2-8°C
|
* If you are not planning to use the kit for over 3 months, store Diethylstilbestrol Standards, Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C or in a freezer.
Sensitivity
|
Sample Type
|
Detection Limit (ng/g or ppb)
|
|
Meat/fish
|
0.075
|
|
Urine
|
0.15
|
|
Feed
|
0.15
|