Selection Antibiotics,  Cell Culture

C₂₀H₃₇N₃O₁₃
M.W.: 527.52
Potency: 1109U/mg
Purity (by HPLC): >90%

Hygromycin B, an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.

Working Conc.
Mammalian cells: 50-1000ug/mL
Plant cells: 20-200ug/mL
Bacteria: 20-200ug/mL
Fungi: 200-1000ug/mL

Your appropriate concentration should be tested experimentally!

Store at 4℃