The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of HUMAN(GM-CSF AB)ELISA KIT in samples. Add HUMAN (GM-CSF AB)ELISA KIT to monoclonal antibody Enzyme well which is pre-coated with  HUMAN(GM-CSF AB)ELISA KITmonoclonal antibody, incubation; then, add HUMAN (GM-CSF AB)ELISA KITantibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concenthumanion of the Human Substance HUMAN (GM-CSF AB)ELISA KIT of sample were positively correlated.