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kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to a The ssay the level of HUMAN(CCP)ELISA KIT in samples. Add HUMAN (CCP)ELISA KIT to monoclonal antibody Enzyme well which is pre-coated with  HUMAN(CCP)ELISA KITmonoclonal antibody, incubation; then, add HUMAN (CCP)ELISA KITantibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concenthumanion of the Human Substance HUMAN (CCP)ELISA KIT of sample were positively correlated.