Recognition Site
C↓PyCGPuG
Source Description
Bacillus stearothermophilus HKC
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl.
Reaction Conditions
Incubation at 65 °C
Reaction Buffer
10 mM Tris-HCl (pH 7.5 at 37 °C)50 mM NaCl10 mM MgCl21 mM DTT100 μg/ml bovine serum albumin
Size
2000 Units;500 Units
Storage Buffer
10 mM Tris-HCl (pH 7.5 at 4 °C)50 mM KCl1 mM dithiothreitol0.1 mM EDTA500 μg/ml bovine serum albumin50% (v/v) glycerol
Storage Conditions
Store at -20 °C.
