Human Tumor Marker AFP ELISA Test Kit

 

Tumor Marker Tests:

 

Category No.	Device Name
10101	AFP
10103	CA125
10104	CA153
10105	CA199
10111	NSE
10107	Free β-HCG
10106	CEA
10109	PSA
10108	Free PSA
10110	PSA 45min

 

 

 

AFP ELISA kit

 

 

Name	AFP ELISA Test
Full name	Human AFP (Alpha Fetoprotein) ELISA Test Kit
Category Name	Cancer ELISA kits
Test	96
Method	ELISA method: Enzyme Linked Immunosorbent Assay
Principle	Sandwich Complex
Detection Range	0-300 ng/mL
Sample	20ul serum
Specificity	98.5%
Sensitivity	2.0 ng/mL
Total Time	~80min
Shelf Life	12-14 Months from the manufacturing date

 

 AFP ELISA kit description:

AFP ELISA test kit is an Enzyme Immunoassay test intended for the quantitative determination of AFP concentration in human serum.

Materials Provided with AFP ELISA Kit:
1. Microtiter wells coated with AFP ELISA Antibody
2. Zero buffer 
3. Reference standard set: 0, 5, 20, 50, 150 & 300 ng/ml
4. AFP EIA Kit Enzyme Conjugate Reagent
5. TMB Substrate
6. AFP Elisa Test Stop Solution
7. Wash Buffer Concentrate 50X

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm 

AFP ELISA Kit Background Information:
Elevation of serum AFP to abnormally high values, occurs in several malignant diseases, most notably non-seminomatous testicular cancer and primary hepatocellular carcinoma. In the case of non-seminomatous testicular cancer, a direct relationship has been observed between the incidence of elevated AFP levels and the stage of disease. Elevated AFP levels have also been observed in patients diagnosed with seminoma with non-seminomatous elements, but not in patients with pure seminoma. In addition, elevated serum AFP concentrations have been measured in patients with other noncancerous diseases, including ataxia telangiectasia, hereditary tyrosinemia, neonatal hyperbilirubinemia, acute viral hepatitis, chronic active hepatitis, and cirrhosis. Elevated serum AFP concentrations are also observed in pregnant women. Therefore, the AFP ELISA test kit measurements are not recommended for use as a screening procedure to detect the presence of cancer in the general population. 

AFP ELISA Test Principle:
The AFP ELISA kit is based on a solid phase enzyme-linked immunosorbent assay. The AFP ELISA kit utilizes one anti-AFP antibody for microtiter wells immobilization and another with mouse monoclonal anti-AFP antibody in the antibody-enzyme horseradish peroxidase conjugate solution. In the AFP ELISA kit, the serum is added to the AFP antibody coated microtiter wells and incubated with the Zero Buffer. If human AFP is present in the specimen, it will combine with the antibody on the well. For additional details please refer to the instructions for use.

Notable features of AFP Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results