Optimal temperature range: 20 ℃ – 37 ℃

Protein impurities: < 0.1%

Specific Activity: > 15 IU/mg (heparin as substrate), > 18 IU/mg (heparan sulfate as substrate)

Concentration: 4 IU/mL

Unit Definition: One international unit (IU) of heparinase II is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from heparin or heparan sulfate per minute at 35 ℃ and pH 7.0.

Stability: Expiration of heparinase II is 12 months from manufacturing date frozen at – 20 ℃ in PBS.

Heparinase II is useful in many applications (usually with heparinase I and heparinase III), for example:

1. As research reagent (glycosaminoglycan degradation).
2. For the preparation of di- and oligo-saccharides of heparin and heparan sulfate and the preparation of oligosaccharide libraries.