Xylanase

 

The major role of Habio Xylanase is to eliminate anti-nutrient effect of xylan in animal feed and improve the digestibility of feed, which will increase utilization of unconventional feedstuffs, reduce feed costs and improve feed efficiency.

 

Definition of Activity

One unite (U) of xylanase is defined as the quantity of enzyme, which liberates 1μmol of reducing sugar from 5.0mmol/L xylan solution at37℃and pH5.5 in1min.

 

Anti-nutrient Effect of Xylan

Arabinoxylan (AX) belongs to non-starch polysaccharide (NSP), is one kind of hemicellulose that exists in many plant feeds. Based on the solubility, it can be divided into WEAX (Water-extractable Arabinoxylan, around 25%-30%) and WUAX (Water-unextractable Arabinoxylan, around 70%-75%).

Table 1 Arabinoxylan content in plant feedstuff (%)

Feed stuff	AX (%)	Feed stuff	AX (%)
Corn	3.7-4.4	Soybean meal	6
Wheat	5.5-6.1	Wheat middling and reddog	14-15
Full fat rice bran	9	Rice bran (defatted)	11
Wheat bran	20-21	Rapeseed meal	4
Cotton meal	9	Sunflower seed meal	11

Note: refer to Dierick& Decuypere, 1994; Partridge, 1999

1. Increasing viscosity of the chyme in gastrointestinal tract, reducing the interaction of nutrient and endogenous enzymes, thus slowing down the rate of diffusion of nutrients.

2. Augmenting the chyme moisture and increasing harmful microbial fermentation in hindgut, thus lowering nutrient utilization efficiency and causing diarrhea.

3. Xylan will combine with digestive enzymes (such as intestinal trypsin, lipase) and bile acid, thus reducing the activity of endogenous digestive enzymes in the animal intestinal tract and affecting digestibility.

4. Nutrients (starch, protein, etc.) are enwrapped by Xylan, thus reducing the nutritional value of feed.

 

Products Functions

1. Degrading xylan to xylooligosaccharide specifically, thus decreasing the viscosity of intestinal contents.

2. Balancing intestinal flora to improve animal immunity and reduce diarrhea ratio.

3. Increasing the diffusion rate of endogenous digestive enzymes, thus improving the nutrients digestibility.

4. Breaking down the structure of cell walls and releasing the nutrients inside, thus increasing the nutrients utilization efficiency of feed raw material nutrients.

5. Improving and ensuring the feed quality.

6. Improving the animal production performance to increase customer profits.

 

Product Features

1.        Origin from two kinds of bacteria strains, having different enzymic characteristics and different activity distribution; the advantage of high specific activity and high stability complement each other.

2.        The combination of high activity of endo-xylanase and exo- xylanase efficiently acts on soluble and insoluble xylan at the same time with high efficiency of hydrolysis.

3.        Better thermostability than general fungal xylanase. The relative enzyme activity remains over 80% at80℃of pelleting temperature.

4. A broad pH range of activity, thus helping play a role more effectively in the gastrointestinal tract of different animals.

5. Strong resistance to digestive enzymes, less negative effect from metal ions, and high storage stability.

 

 

Graph1 Habio Xylanase pH Curve

 

Graph2 Habio Xylanase Thermostability (water bath for 5min)

 

 

 

 

 

 

 

 

 

 

 

Table2. The Effect of Metal Ion on the activity of Habio Xylanase

Metal Ion	Concentration C/mol.L-1	Relative activity/%	Evaluation
Fe2+ Ca2+ Mn2+ Cu2+ Zn2+ Control group	10-3 10-3 10-3 10-3 10-3 10-3	101.21 106.09 96.51 93.87 87.76 100	0 + + 0 - 0

Note: + refers to activation; - refers to inhibition; 0 refers to no obvious impact (the effect on enzyme activity is within 10%).

 

Specifications and Usage

 

 

Package and Storage for all products

Solid products: 25kg/Kraft-bag or 20kg/Barrel;

Liquid products: 30kg/Plastic barrel or 225kg/Plastic barrel.

Store in original package under sealed, room temperature condition for 12 months to solid form, and 6 months to liquid form (less than25 ℃).

Avoid to be exposed to the sun, rain, high temperature and high humidity.

Technical Service

Offer Xylanase usage formula, enzyme activity test and related product technical service to ensure the using result.