High Purity Trypsin inhibitor Recombinant Aprotinin 6000KIU/mg pro / Aprotinin Trasylol 9087-70-1

 

Test	Specifications
Description	Almost white hygroscopic powder
Identification	Positive of inhibition of trypsin
Absorbance	λmax=277nm Absorbance at 15.0 EPU/ml ≤0.8
Impurities A	≤8.0 %
Impurities B	≤7.5 %
Impurities C	≤1.0 %
Aprotinin oligomers	≤1.0 %
Loss on drying	≤6.0 %
Abnormal toxicity	conform
Histamine	≤0.2 μg / 3 E.P.U
Bacterial endotoxins	≤0.14 IU/E.P.U
Potency	≥3.0 E.P.U / mg

 

 

 

Synonyms
Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP.

Introduction
Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. Aprotinin is present in blood and in most tissues, with a high concentration in lung. Aprotinin inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins (e.g., CD11b).

Description
Aprotinin is a natural proteinase inhibitor polypeptide consisting of fifty-eight amino acids {C 284 H 432 N 84 O 79 S 7} arranged in a single polypeptide chain, cross-linked by three disulfide bridges and having a molecular mass of 6512.

Source
Bovine Lung

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation
The protein (1mg/ml) was lyophilized with no additives.

Solubility
It is recommended to reconstitute the lyophilized Aprotinin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Stability
Lyophilized Aprotinin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Aprotinin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Unit Definition
1 Unit corresponds to 1 biological kallikrein inhibitor unit (KIU)
1 TIU ?1,300 KIU

Activity
6,462 KIU (Kallikrein Inactivator Units) per mg, 4.97 TIU/mg.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.