This kit allows for the determination of C.JEJUNI expression in human serum, and other
biological fluids.
The kit assay C.JEJUNI level in the sample, use Purified C.JEJUNI antibody to coat
microtiter plate wells, make solid-phase antibody, then add C.JEJUNI to wells,
Combined With C.JEJUNI, after washing and removing non-combinative antibody and
other components ,then Combined C.JEJUNI antibody
which with HRP labeled
become antibody – antigen - enzyme- antibody complex, after washing Completely,
Add TMB substrate solution,, TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution
and the color change is measured spectrophotometrically at a wavelength of 450 nm.
Compared with the CUTOFF value, according to this to judge C.JEJUNI exist in the
sample or not