| Purity: | 90 |
|---|---|
| Brand Name: | Pars Biochem |
| Grade Standard: | Medicine Grade |
| Type: | Biochem |
| CAS No.: | 83-33-0 |
| Usage: | Animal Pharmaceuticals |
| Place of Origin: | China (Mainland) |
Quick Details
Specifications
| Details: |
Overview
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type: serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range: 50pg/ml-3000 pg/ml
Kit Components: Assay plate (12 × 8 coated Microwells) ……………………..1
Standard: 3600 pg/ml……………………………………… 1×0.5ml
Standard Diluent…………………………………………… 1×1.5ml
HRP-Conjugate Reagent …………………………………… 1×6ml
Sample Diluent……………………………………………… 1×6ml
Chromogen Solution A…………………………………………1×6ml
Chromogen Solution B…………………………………………1×6ml
Stop Solution …………………………………………………1×6ml
Wash Solution…………………………………………………1×20ml×30 fold
User manual……………………………………………………1
Adhesive Strip……………………………………………………2
Product Principle: the kit is for the quantitative level of Amylase in the sample, adopt purified Human Amylase antibody to coat microtiter plate, make solid-phase antibody, then add Amylase to wells, Combine Amylase antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of Amylase in the samples is then determined by comparing the O.D. of the samples to the standard curve..
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