Principle

Bisphenol A (BPA) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Bisphenol A in tissue,honey, milk, urine and Serum.

the coupling antigens are coated on the micro-well stripes. the Bisphenol A in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Bisphenol A antibodies. after the addition of the enzyme conjugate, the TMB substrate is added for coloration.

the optical density (OD) value of the sample has a negative correlation with the Bisphenol A in it. the measurement is made photometrically at 450 nm. the absorption is inversely proportional to the Bisphenol A concentration in the sample.

 

Technical specification:

Sensitivity: 5 ppb      Detect time: Approx.75 min

 

Detect limit

Urine,serum.................................... 10 ppb

Liver,tissue......................................7.5 ppb

 

Recovery rate

Urine,serum................................ 70 ± 10%    

Liver,tissue..................................80 ± 10%

 

Cross-reaction rate

Bisphenol A .......................100%

Thiamphenicol........................< 0.1%

Florfenicol...............................< 0.1%

 

Components

1. Microplate: 12 strips with 8 removable wells each

2. 6× standard solution (1 mL each):

     0 ppb, 1ppb, 5 ppb, 15 ppb, 45 ppb, 135 ppb, 405 ppb,

3. Enzyme conjugate (12 mL)........................................................ red cap

4. Antibody working solution (7 mL)..........................................Green cap

5. Substrate  A solution (7 mL)................................................. White cap

6. Substrate B solution (7 mL).................................................... Black cap

7. Stop solution (7 mL).............................................................. Yellow cap

8. Washing solution  (40 mL, 20× )  ............ .................. transparent cap

9. Redissolving solution (50 mL, 2× ).......................................... blue cap