Test Principle

Bluetongue Antibody ELISA Kit is indirect competitive enzyme linked immunosorbent assay designed to detect the presence of antibody to goat, sheep , bovine bluetongue in the serum.  A microtitration format has been configured by coating bluetongue antigen in alternating columns on the plate. upon incubation of the test sample in the coated well, antigens specific to pox forms a complex with the coated viral antibodies. after washing away unbound material from the wells, an anti-porcine horseradish peroxidase conjugate is added that binds to any bluetongue antibodies attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate is added to the wells. Stop the reaction by adding stop solution. Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength if available.

Enzyme immunoassays for the qualitative of pox antibodies against goat, sheep ,bovine in the serum.

 

Materials Supplied

1  Microtiter Plate	1 pieces (96 wells)
2  Negative control	1 tube (0.8mL)
3  Positive control	1 tube (0.8mL)
4  Enzyme(HRP) Conjugate	1 bottle (12 mL)
5  WashingSolution	1 bottle(10×,100 mL)
6  Substrate A	1 bottle(6 mL)
7  Substrate B	1 bottle(6 mL)
8  Stop solution	1 bottle(6 mL)
9  Sample diluent	1Bottle (2×,100 mL)