PRINCIPLE

Bovine Tuberculosis (TB) is a serious bacterial disease caused by Mycobacterium bovis that affects the respiratory system. Bovine TB, capable of infecting most mammals including humans, is a chronic disease that has affected animal health throughout recorded history.

the Bovine TB antibody ELISA is an indirect Enzyme Linked Immunosorbent Assay for the qualitative detection of Mycobacterium bovis antibody in serum.

the Bovine TB Ab ELISA kit contains a microplate pre-coated with Bovine TB antigen. during the first incubation, Bovine antibodies bind to the antigens in the wells, if present in the test sample. Following this incubation, all unbound material is removed by aspiration and washing. HRP conjugate added subsequently forms a complex with the bovine antibodies, while unbound materials are removed by aspiration and washing before the addition of a substrate solution. Thus, the residual enzyme activity found in the well is directly proportional to the anti-Bovine TB antibody concentration in specimens. this activity is detected by incubation  with a substrate solution. the reaction is stopped by addition of stop solution and colorimetric reading performed at 630nm using a spectrophotometer within 15 minutes.

Specially selected Bovine TB antigens are used as detector materials in the test. It allows detection of the Bovine TB antibodies in specimens with a high degree of accuracy.

 

MATERIALS SUPPLIED

1  Microtiter Plate	1 pieces (96 wells)
2  Negative control	1 tube
3  Positive control	1 tube
4  Enzyme Conjugate	1 bottle
5  Washing buffer	2 bottle
6  Substrate A	1 bottle
7  Substrate B	1 bottle
8  Stop solution	1 bottle