Test Principle

Canine Hydatid Coproantigen ELISA Kit is indirect competitive enzyme linked immunosorbent assay designed to detect the presence of antigen to canine Hydatid Coproantigen in the canine stool. A microtitration format has been configured by coating canine Hydatid antibody in alternating columns on the plate. upon incubation of the test sample in the coated well, antigens specific to canine Hydatid forms a complex with the coated viral antibodies.after washing away unbound material from the wells, an anti-porcine horseradish peroxidase conjugate is added that binds to any canine antigens attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate is added to the wells. Stop the reaction by adding stop solution. Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength if available.

Enzyme immunoassays for the qualitative of antigens against canine Coproantigen in the stool samples.

 

Materials Supplied

1 Microtiter Plate	1 pieces (96 wells)
2 NegativeCOntrol	1 tube (0.8mL)
3 PositiveCOntrol	1 tube (0.8mL)
4  Enzyme Conjugate	1 bottle (12 mL)
5 WashingSOlution	1 bottle(10×,100 mL)
6 Substrate A	1 bottle(6 mL)
7 Substrate B	1 bottle(6 mL)
8 Stop Reaction SOlution	1 bottle(6 mL)
9 SampleDIluent	1Bottle (2×,100 mL)