1. Principle

 Erythromycin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of  Erythromycin  in milk, honey and meat.

 the coupling antigens are coated on the micro-well stripes. the Erythromycin in the sample  and the coupling antigens coated on the micro-well stripes compete for the  

 anti-Erythromycin antibodies. after the addition of the enzyme conjugate, the TMB substrate is added for coloration. the optical density (OD) value of the sample has a  negative correlation with the Erythromycin in it. the measurement is made photometrically at 450 nm. the absorption is inversely proportional to the Erythromycin    concentration in the sample.

 

2.  Technical specification:

Sensitivity:0.1 ppb        Detect time:Approx.75 min

 

Detect  limit:

Chicken,duck, pork, shrimp,fish,honey and milk.................4 ppb  

 

Cross-reaction rate:

Erythromycin...................................................................... 100%

Erythromycin Thiocyanate................................................ 105%

Erythromycin Ethylsuccinate............................................ 85%

 

Recovery rate:

Tissue ....................................................................... 85 ± 20%    

Honey, milk ............................................................. 75 ± 20%

 

3. Components

1)Microplate: 12 strips with 8 removable wells each

2)6× standard solution (1 mL each): 0ppb,0.1 ppb,0.3 pb,0.9 ppb,2.7 ppb,8.1ppb

3)Enzyme conjugate (12 mL) ................................................ red cap

4)Antibody solution (7 mL)  .............................................. green cap

5)Substrate  A solution (7 mL) .............................................. white cap

6)Substrate B solution (7 mL) ................................................... Black cap

7)Stop solution (7 mL)................................................................ Yellow cap

8)Washing solution  (40 mL, 20× Concentrate).........................Transparent cap

9)Buffer 1 (50 mL, 2× Concentrate)............................................Blue cap