Description:
Choline is usually grouped with vitamin B family. It can accelerate fat metabolism in the liver and kidney and it is the basis of body's synthesis of acetylcholine, affecting the transmission of nerve signals. Additionally, choline is a major resource of methyl imperative in the formation of methionine in vivo.
-white color, superiorr quality of choline chloride liquid
-no moisture absorbtion again
-suitable for fish feed addition
-the product sells higher than other 50% product with plant carriers because of a higher cost of silica carrier but currently it is fairly workable adding form among all powder products

 Product Introduction 
Molecule Formula:HOCH2CH2N(CH3)3Cl
Melting Point(℃): 302～305(decomposed)
Content of Choline Chloride:50%, 60% HG/T2941-2004
Density:0.35-0.65g/cm3 Q/HEX05-2000
Size of Particle: 95% under 60 mesh screen Q/HEX05-2000
Loss on heating:4% HG/T2941-2004
Appearance: white powder with strong hygroscopic and slight fishy smell
Toxicity: LD50(mg/kg);  6640 through rats' month

 Product Application:
    Choline, a member of vitamin B family, can accelerate fat metabolism of liver and kidney and affect the transmission of nerve signals as the basis of synthesis of acetylcholine in bodies. and choline is the major resource of methyl to synthesize methionine in vivo. Although many foods contain natural choline, its intake is still far from increasing demand for faster growth of animals for modern husbandry industry. but choline products are being expected to solve the problem. The deficiency of choline can cause fatty liver, slow growth, poor egg productivity, high death rate etc..

Chemical Testing Methods:
Molecular Formula: HOCH2CH2N(CH3)3Cl
Molecular Weight: 139.36
1. International Non Aqueous Titration
Powder: Weight 0.7g(50% choline chloride) sample of 3 hours dried under 80℃, put in triangle flask. Add 40ml methanol(CH4O), with 30 minutes fully shake and blend, then filter. Rinse out and sludge three times separately with 20ml, 15ml, 15ml methanol. Combine filtrate with lotion, evaporate until dry, use 20ml glacial Acetic Acid to resolve, 2ml Anhydride Acetate, 10ml Hg Acetate and two drops of crystalline purple indicatory potion, use Perchloric Acid(HCIO4) standard solution (concentration is 0.1mol/L) titration to pure blue, simultaneously make lacuna.
Liquid: Weight 0.6g(70%choline chloride) sample, add 20ml glacial Acetic Acid to, 2ml Anhydride Acetate, 10ml Hg Acetate and two drops of crystalline purple indicatory potion, use Perchloric Acid(HCIO4) standard solution (concentration is 0.1mol/L) titration to pure blue, simultaneously make lacuna.
     Content of Choline Chloride = C×(V-V. )×139.63)/(m×1000) 
 C-Perchloride Chloride standard solution's concentration,mol/L;  
 V-Perchloride Chloride titration quantum,ml;  
 V. -blank titration quantum,ml;  
       139.63-Chloric Ion's mol quality,g/mol.
2. Chloric Ion Testing Method
Liquid: Weight 0.5g sample, put in 250ml Iodic Measuring flask, add 50ml water and shake until equable, add 0.5ml Kalium Chromic Acid (K2CrO4), use Silver Nitrate(AgNO3) standard solution nitration to brick red, simultaneously make lacuna.
Powder: Weight 1.4g sample of 3 hours dried under 80℃, put in a 100mL conical flask, with the aid of water. Stand for 20 minutes, filter it. Discard 20mL of the primary filtrate, pipet 50mL of filtrate to a 250ml of Iodic Measuring flask, follow the step of liquid test. Content of Choline Chloride = C×(V-V. )×139.63)/(m×1000)
C-Silver Nitrate standard solution's concentration,mol/L;  
V-Silver Nitrate titration quantum,ml;  
V. -blank titration quantum,ml;  
139.63-Chloric Ion's mol quality,g/mol.
3. Sodium Tetraphenylborate Testing Method
Weight about 1g sample(exact weight is 0.0002g), put in 100ml capacity flask, add water to resolve and dilute until graduation, shake equally, filter, exactly take 10ml to put in 100ml beaker, add 20ml water, one drop Aluminum Oxide (10%),20ml Sodium Tetraphenylborate(2%, pure Sodium Tetraphenylborate 2g in good class, add water to resolve and dilute until 100ml, add two drops of 0.5mol/L NaOH, put in 24h after shake equally, filter, expiry date of this solution is 5d), continuously shake 30 minutes, filter with weight no. 4 crucible which is dried by heat in advance, wash beaker with water four times, 10ml every time, and filter filtrate together with, sludge and dry by heat 2h under 105℃, take it out and put in desiccator for cool off, weight.
4. Reineckate Method
Weight about 1g sample which has been dried to the constant weight. Put the sample in a 250ml of conical flask, add 70mL of water to resolve, shake equally, filter, heat the sample at 70℃ for 15 minutes. Shake the solution in the vibrator for 10 minutes, with the aid of water. Filter it and exactly take 25ml of the filtrate to a 100ml beaker, add 3mL of 400g/L NaOH solution, heat it on an electric cooker until boiling about 5 minutes, cooling it. Then follow the step of liquid test.

Content of Choline Chloride =(m2-m1)/m0×3.3045×100%
M0-the weight of sample, g; 
M2-the weight of sample and crucible filter;
M1-the weight of crucible filter.
5. Nitrogen Ion Testing Method
1.  Silica carrier
Weight 0.5g, 0.6g sample use Nitrogen Ion Testing Method to mensurate content of nitrogen 
Content of Choline Chloride = mensurative Choline Chloride's Nitrogen content/pure Choline Chloride content(10.03) X 100%
2.   Corn Cob carrier
Weght 1g sample, put in funnel with white nature determined filter paper, overwash ten times with water, take filter paper and dry by heat, use Nitrogen Ion Testing Method to mensurate content of Nitrogen (N1), simultaneously weight 0.5g-0.6g and directly measure content of Nitrogen (N2).
Content of Choline Chloride =(N2-N1)/10.03×100%
N2-Directly tested content of Nitrogen
N1-Residue(carrier)'s Nitrogen content;  
10.03-Content of pure Choline Chloride