AG-CFDNA Column provides a simple and reliable method for the rapid preparation of High-quality circulating cell-free DNA from serum, plasma, amniotic fluid, Cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic Methods are used to efficiently recover total DNA (including cell-free Apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of Sample (Reference Figure A).
DNA Recovery |
Recover DNA 100bp (optimized for recovery of Cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. the yield Varies depending on the sample source and the health of the donor. |
Equipment |
Water bath or heat block (55C), microcentrifuge, Vacuum/vacuum manifold or swinging bucket centrifuge. |
Sample Sources |
Serum, plasma, amniotic fluid, and cerebrospinal Fluid (CSF). |
DNA Purity |
High-quality DNA is ready for all sensitive Downstream applications such as qPCR and next-Generation sequencing. |
Storage |
Eluted DNA should be stored at -20C. |
Recovery Volume |
35 l of DNA Elution Buffer or DNase free water. |
Processing Volume |
Plasma Single centrifugation: up to 3 ml |
