AG-CFDNA Column Set provides A simple and reliable method for the rapid preparation of high-quality Circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal Fluid (CSF), and saliva. A combination of chemical and enzymatic methods are Used to efficiently recover total DNA (including cell-free apoptotic, necrotic, Mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A).
the AG-CFDNA Column Set Contains with
20ml Funnel Column
CFDNA Mini Column
Collection Tube III
50ml Centrifuge Tube
DNA Recovery |
Recover DNA 100bp (optimized for Recovery of cell-free DNA). Yields can vary considerably among different Individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or Serum. the yield varies depending on the sample source and the health of the Donor. |
Equipment |
Water bath or heat block (55C), Microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge. |
Sample Sources |
Serum, plasma, amniotic fluid, and Cerebrospinal fluid (CSF). |
DNA Purity |
High-quality DNA is ready for all Sensitive downstream applications such as qPCR and next-Generation Sequencing. |
Storage |
Eluted DNA should be stored at -20C. |
Recovery Volume |
35 l of DNA Elution Buffer or DNase free water. |
Processing Volume |
Plasma Single centrifugation: up
to 3 ml |
