Parameters

Ligand

-NTA-Ni²⁺

Matrix

6% Highly cross-linked spherical agarose

Average particle size

90 μm

Dynamic binding capacity

~50 mg (histidine)₆-tagged protein(30kD)/mL

Ligand density

~15 μmol/mL

Max. Operating pressure

0.3 MPa

Max. Flow velocity

≤600 Cm/h *

PH stability

2-14 (Short Term, CIP)

4-13 (Long Term)

Chemical stability (without Ni²⁺)

Stable in all commonly used buffers. 1 M NaOH, 70% acetic acid, 2% SDS, 30% 2 -propanol.

Storage

20%Ethanol

Storage temperature

4-28^oC