| Brand Name: | Purose |
|---|---|
| Appearance: | Blue gelatinous |
| Purity: | 99% |
| Grade Standard: | Medicine Grade |
| CAS No.: | 9012-36-6 |
| Place of Origin: | China (Mainland) |
Quick Details
Specifications
Ni-TED Purose 6 Fast Flow has the following advantages:
Stable performance
Tolerance to EDTA and DTT. the most excellent selection for cell culture samples
Tolerance to NaOH, with no Need for taking off Ni2+. A time saving on CIP
Negligible Ni2+Leakage
Fast flow velocity
Reliable scale-up
Available in convenient PrePacked Column formats
Parameters | |
Ligand |
-TED-Ni2+ |
Matrix |
6% Highly cross-linked spherical agarose |
Average particle size |
90 μm |
Dynamic binding capacity |
10-15 mg (histidine)6-tagged protein/mL |
Ligand density |
60-100 μmol/mL |
Max. Operating pressure |
0.3 MPa |
Max. Flow velocity |
≤600 Cm/h * |
PH stability |
2-14 (Short Term, CIP) 4-13 (Long Term) |
Chemical stability |
Stable in all commonly used buffers. 0.01 M NaOH or HCl for 1 week, 10 mM EDTA, 5 mM DTT, 5 mM TCEP, 20 mM Β-mercaptoethanol, 1 M NaOH, and 6 M guanidine-HCl for 24 hours, 30% 2-propanol for 20 mins. |
Storage |
20%Ethanol |
Storage temperature |
4-28oC |
* XK 50/30 column with 15 cm bed Height, 1 bar, 25oC, Test with deionized water.
