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Quick Details

Brand Name: Purose
Appearance: Blue gelatinous
Purity: 99%
Grade Standard: Medicine Grade
CAS No.: 9012-36-6
Place of Origin: China (Mainland)

Specifications

Ni-IDA Purose 6 Fast Flow has the following advantages:

Stable performance

Very high protein binding Capacity

Fast flow velocity

Reliable scale-up
Available in convenient PrePacked Column formats

Parameters

Ligand

-IDA-Ni2+

Matrix

6% Highly cross-linked spherical agarose

Average particle size

90 μm

Dynamic binding capacity

~40 mg (histidine)6-tagged protein/mL

Ligand density

~15 μmol/mL

Max. Operating pressure

0.3 MPa

Max. Flow velocity

≤600 Cm/h *

PH stability

2-14 (Short Term, CIP)

4-13 (Long Term)

Chemical stability (without Ni2+)

Stable in all commonly used buffers. 1 M NaOH, 70% acetic acid, 2% SDS, 30% 2 -propanol.

Storage

20 %Ethanol

Storage temperature

4-28oC

* XK 50/30 column with 15 cm bed Height, 1 bar, 25oC, Test with deionized water.


NiIDA Purose 6 Fast Flow is a Ni2 ions immobilized affinity chromatography medium