Catalog Number:RCPB01

CAS: 9025-24-5
EC:3.4.17.2

Storage temperature:2-8℃

Source:Recombinant carboxypeptidase B is a genetically engineered protein expressed inE.Coli 

Protein Sequence:the amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

 

1.DESCRIPTION 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. the molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. the enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into  E.Coli for expression with equivalent properties compared to animal original carboxypeptidase B.Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

  

2.MAIN FEATURES 

Source	RecombinantE. Coli
Appearance	White or off white, or yellowish powder
Specific activity	≥170 units/mg pro.
Purity(SDS-PAGE)	Single major band
Molecular Weight(SDS-PAGE)	33.8±3.4 kDa

UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

  

3.RECOMMEND USAGE  

Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. the ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0. 

  

4.STABILITY of STORAGE and TRANSPORT 

Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.after dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: the product is stable by blue ice insulation transport.

  

5.PRODUCT ADVANTAGE 

Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues is less than the limits of biological products.

Lyophilized powder:the product is lyophilized powder and is convenient to store and transport.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers' requirement.

  

6.PRODUCT USAGE 

the quality test of recombinant antibodies; the determination of C-terminal amino acids of proteins; the production of other recombinant polypeptides; and the enzymatic synthesis of certain specific compounds.

  

7.RELATED PRODUCTS 

Recombinant trypsin

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase