Purity: | 100% |
---|---|
Brand Name: | Yaxinbio |
Grade Standard: | Medicine Grade |
Type: | |
CAS No.: | 9002-07-7 |
Quick Details
Specifications
Catalog Number:RHT03
CAS: 9002-07-7
EC:3.4.21.4
Storage temperature:2-8℃
Source:Recombinant human trypsin is a genetically engineered protein expressed inE.Coli
Protein Sequence:the amino acid sequence of recombinant human trypsin is identical to human pancreas-derived trypsin
1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant human trypsin is a genetically engineered protein expressed inE.Coli and the amino acid sequence is identical to human pancreas-derived trypsin,with equivalent properties compared to native trypsin.the enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
2.MAIN FEATURES
Source |
RecombinantE. Coli |
Appearance |
White or off white powder |
Specific activity |
≥2500 USP units/mg pro. |
Protein Content |
≥ 90% |
Purity(HPLC) |
≥ 95% |
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.the ratio to aimed protein is 1:50 to 1:1000 (w/w).the optimum pH is pH7.0-11.0.
4.STABILITY of STORAGE and TRANSPORT
Stability of storage:Recombinant human trypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.after dissolved with 1mM HCl, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: the product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
Animal origin free: Recombinant human trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder:the product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory Requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers' requirement.
6.PRODUCT USAGE
Recombinant human trypsin is an endopeptidase that can be used for lysine and arginine C-terminal cleavage of peptide bonds to cleave macromolecules into small peptides. Recombinant human trypsin is widely used in a variety of biotechnological processes, such as: cell culture cell separation of various tissues; degradation of denatured proteins; enzymatic hydrolysis, sequencing; stem cell therapy; tumor cell therapy.
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant trypsin
Recombinant enterokinase