Feiyue's Human EDN1(Endothelin-1) ELISA Kit is an ELISA reagent for detection of EDN1(Endothelin-1), plasma or cell with sensitivity, specificity and consistency.

Human EDN1(Endothelin-1) Introduction
EDN1 is a potent vasoconstrictor that in humans is encoded by the EDN1 gene and produced by vascular endothelial cells. The protein encoded by this gene is proteolytically processed to release a secreted peptide termed EDN1. EDN1 is one of three isoforms of human endothelin.

Human EDN1(Endothelin-1) ELISA Kit Test Method
this kit uses sandwich ELISA to detect the concentration of Human EDN1.
Human EDN1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human EDN1 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.the concentration of Human EDN1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

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