Rat OVA sIgE(Ovalbumin specific Immunoglobulin E) ELISA Kit Basic Information 

Product Name	Rat OVA sIgE(Ovalbumin specific Immunoglobulin E) ELISA Kit
Catalog NO.	FY-ER4174
Alias	OVA sIgE
Application	OVA sIgE ELISA Kit allows for the in vitro quantitative determination of OVA sIgE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size	48T, 96T
Storage	2-8 ℃ for 6 months
Sensitivity	<0.469ng/ml
Species	Rat
CV (%)	Intra-Assay: CV<8% Inter-Assay: CV<10%
Note	for Research Use Only

Feiyue's Rat OVA sIgE is an ELISA reagent for detection of OVA sIgE in serum, plasma or cell with sensitivity, specificity and consistency.

Rat OVA sIgE(Ovalbumin specific Immunoglobulin E) Introduciton

Immunoglobulin E (IgE) is a type of antibody (or immunoglobulin (Ig) "isotype") that has been found only in mammals. IgE is synthesised by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing four Ig-like constant domains (Cε1Cε4). IgE is thought to be an important part of the immune response against infection by certain parasitic worms, including Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE is also utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum. IgE may have evolved as a defense to protect against venoms. Ovalbumin (OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin is an important protein in several different areas of research and commonly used to stimulate an allergic reaction in mice.

Rat OVA sIgE(Ovalbumin specific Immunoglobulin E) ELISA Kit Test method

this kit was based on indirect enzyme-linked immune-sorbent assay technology. 

Antigen was pre-coated onto 96-well plates. and the HRP conjugated antibody was used as detection antibodies. the standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. the density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. Absorbance at 450nm in a microplate reader, and then the concentration of CRP can be calculated.