Human OXA(Orexin A) ELISA Kit Basic Information 

Product Name	Human OXA(Orexin A) ELISA Kit
Catalog NO.	FY-EH4175
Alias	OXA
Application	OXA ELISA Kit allows for the in vitro quantitative determination of OXA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size	48T, 96T
Storage	2-8 ℃ for 6 months
Sensitivity	<37.5pg/ml
Species	Human
UniProt ID	O43612
CV (%)	Intra-Assay: CV<8% Inter-Assay: CV<10%
Note	for Research Use Only

Feiyue's Human OXA is an ELISA reagent for detection of Human OXA in serum, plasma or cell with sensitivity, specificity and consistency.

Human OXA(Orexin A) Introduciton

Orexin-A, also known as hypocretin-1, is a naturally occurring neuropeptide and orexin isoform. the orexinergic nucleus in the lateral hypothalamus is the primary orexin projection system in the brain. Orexin-A is a peptide composed of 33 amino acids including an N-terminal pyroglutamyl residue and two intramolecular disulfide bridges between cysteine residues in 6 and 12 and 7 and 14 positions. Orexins are highly excitatory neuropeptides that were first discovered in the brains of rats. It is a peptide that is produced by a very small population of cells in the lateral and posterior hypothalamus. Orexins strongly excite various brain nuclei (neurons) to affect an organism's wakefulness by affecting their dopamine, norepinephrine, histamine and acetylcholine systems.

Human OXA(Orexin A) ELISA Kit Test method

this kit uses Competitive-ELISA to detect Human OXA. 

the microtiter plate provided in this kit has been pre-coated with target. during the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme -substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.