| Place of Origin: | China (Mainland) |
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Specifications
Mouse OPN(Osteopontin) ELISA Kit Basic Information
Feiyue's Mouse OPN is an ELISA reagent for detection of OPN in serum, plasma or cell with sensitivity, specificity and consistency.
Mouse OPN(Osteopontin) Introduciton
Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). the murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. OPN is a highly negatively charged, extracellular matrix protein that lacks an extensive secondary structure. It is composed of about 300 amino acids (297 in mouse; 314 in human) and is expressed as a 33-kDa nascent protein; there are also functionally important cleavage sites.
Mouse OPN(Osteopontin) ELISA Kit Test method
this kit uses sandwich ELISA to detect the concentration of Mouse OPN.
Mouse OPN-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse OPN antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. the concentration of Mouse OPN in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
Product Name |
Mouse OPN(Osteopontin) ELISA Kit |
Catalog NO. |
FY-EM4170 |
Alias |
OPN; Osteopontin; Eta-1; Spp1; BNSP; Bone sialoprotein 1; MGC110940; Nephropontin; secreted phosphoprotein-1; osteopontin; bone sialoprotein; SPP-1; SPP1; CALPHA1 fusion; Urinary stone protein; uropontin |
Application |
OPN ELISA Kit allows for the in vitro quantitative determination of OPN concentrations in serum, plasma, tissue homogenates and other biological fluids. |
Size |
48T, 96T |
Storage |
2-8 ℃ for 6 months |
Sensitivity |
<23.438pg/ml |
Species |
Mouse |
UniProt ID |
P10923 |
CV (%) |
Intra-Assay: CV<8% |
Note |
for Research Use Only |
Feiyue's Mouse OPN is an ELISA reagent for detection of OPN in serum, plasma or cell with sensitivity, specificity and consistency.
Mouse OPN(Osteopontin) Introduciton
Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). the murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. OPN is a highly negatively charged, extracellular matrix protein that lacks an extensive secondary structure. It is composed of about 300 amino acids (297 in mouse; 314 in human) and is expressed as a 33-kDa nascent protein; there are also functionally important cleavage sites.
Mouse OPN(Osteopontin) ELISA Kit Test method
this kit uses sandwich ELISA to detect the concentration of Mouse OPN.
Mouse OPN-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse OPN antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. the concentration of Mouse OPN in the sample can be calculated by comparing the absorbance of the sample with the standard curve.