Product name:Human EDN(Eosinophil-Derived Neurotoxin) ELISA Kit

Catalog NO.:FY-EH4608

Reactivity:Human

Assay range:0.31220ng/mL

Sensitivity:0.132ng/mL

Uniprot ID:P10153

Detection Method:Sandwich ELISA, Double Antibody

Assay Length:3h

Size:48T, 96T

Storage:28 ℃ for 6 months

CV %():Intra-Assay: CV<10%; Inter-Assay: CV<12%

Note: for Research Use Only

EDN(Eosinophil-Derived Neurotoxin) Basic information

the protein encoded by this gene is found in eosinophil granulocytes. It is closely related to the eosinophil cationic protein (RNASE3) from which it diverged ~50 million years ago after the split between the old world and the new world monkeys. It is relatively neutral and has cytotoxic properties. It is capable of reducing the activity of single strand RNA viruses in culture through its enzymatic activity. It also serves as an attractant to immune cells.

Human EDN(Eosinophil-Derived Neurotoxin) ELISA Kit test method

Feiyue's Human EDN(Eosinophil-Derived Neurotoxin) Elisa kit is an ELISA reagent for detection of Neutrophil elastase in Serum, plasma, tissue homogenates and other biological fluids.

this kit uses sandwich ELISA to detect the concentration of EDN(Eosinophil-Derived Neurotoxin), EDN(Eosinophil-Derived Neurotoxin) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standard samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by an additional anti- EDN(Eosinophil-Derived Neurotoxin) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. the concentration of EDN(Eosinophil-Derived Neurotoxin) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1." Sequence of human eosinophil-derived neurotoxin cDNA: identity of deduced amino acid sequence with human nonsecretory ribonucleases."

2." Eosinophil cationic protein cDNA. Comparison with other toxic cationic proteins and ribonucleases."

3." Molecular cloning of the human eosinophil-derived neurotoxin: a member of the ribonuclease gene family."

4." Structure and chromosome localization of the human eosinophil-derived neurotoxin and eosinophil cationic protein genes: evidence for intronless coding sequences in the ribonuclease gene superfamily."