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Quick Details

Place of Origin: China (Mainland)

Specifications

Human on(Osteonectin) ELISA Kit Basic Information 

Product Name

Human on(Osteonectin) ELISA Kit

Catalog NO.

FY-EH4164

Alias

on; BM-40; Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Secreted protein acidic and rich in cysteine; secreted protein; acidic; cysteine-rich; osteonectin

Application

on ELISA Kit allows for the in vitro quantitative determination of ON concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

48T, 96T

Storage

2-8 ℃ for 6 months

Sensitivity

<0.094ng/ml

Species

Human

UniProt ID

P09486

CV (%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

for Research Use Only


Feiyue's Human on is an ELISA reagent for detection of ON in serum, plasma or cell with sensitivity, specificity and consistency.
Human ONOsteonectin ELISA Kit
Human on(Osteonectin) Introduciton
Osteonectin (ON) also known as secreted protein acidic and rich in cysteine (SPARC) or basement-membrane protein 40 (BM-40) is a protein that in humans is encoded by the SPARC gene. Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over-expression and ampullary cancers and chronic pancreatitis has been found.

Human on(Osteonectin) ELISA Kit Test method
this kit uses sandwich ELISA to detect the concentration of Human ON.
Human on-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human ON antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. the concentration of Human ON in the sample can be calculated by comparing the absorbance of the sample with the standard curve.