Full name: Staphylococcus aureus V8

Alias: V8 protease

CAS No.: 66676-43-5

EC No.: 3.4.21.19

Description: V8 protease is a serine protease that specifically hydrolyzes the peptide bond on the carboxy side of aspartic acid and glutamic acid residues. the optimum pH was 8.0 8.5. Enzyme inhibitors include diisopropyl fluorophosphate (DFP) α 2-macroglobulin and n α=- P-toluenesulfonyl-l-lysine chloromethyl ketone (TLCK).

Unit definition: 37 ℃, pH7.8, catalytic hydrolysis 1 per minute μ Mol z-phe-leu-glu-4-nitranilide is one unit of enzyme activity.

Uses: used for protein structure and sequence analysis.

Specific activity: at 25 ° C, the activity measured with z-phe-leu-glu-4-nitranilide as substrate is about 20 U / Mg (at 37 ° C, the activity measured with casein as substrate is about 500 U / mg).

Specification: 50 g / piece, 1U / piece

Storage: - 20 ° C