BroadDye^TM TerminatorV3.5Cycle Sequencing Kit

 

 

theBroadDye^TM TerminatorV3.5Cycle Sequencing Kit provides pre-mixed reagents for Sanger sequencing reactions.

 

Description:

this kit uses four different fluorescent dyes to label ddNTPs, which are added sequentially to the primer through a cycle sequencing reaction. the kit reagents are suitable for performing fluorescence-Based cycle sequencing reactions on single-stranded or double-stranded DNA templates, on PCR fragments, and on large templates (for example, BAC clones).

BroadDye^TM terminator kit is compatible with commercially available BigDye terminator V3.1 kit, without any changes in the workflow.

 

FEatures:

with enhanced robustness and better dye mobility characteristics, the BroadDye^TM TerminatorV3.5Cycle Sequencing Kit can be used in a wide range of applications with the following features:

 Long read lengths.

 Better performance reading through GC rich regions.

 Longer reads with more uniform peak heights.

 Improved productivity for less cost.

Applications:

 De novo sequencing

 Resequencing

 Sequencing difficult templates

 Long-read sequencing

 Sequencing across all template types (plasmids, PCR products)

 Mixed-base detection

 Sequencing short PCR products using rapid electrophoresis run modules

 

BroadDye^TM TerminatorV3.5Cycle Sequencing Kit contents-Standard

Contents	QuantitySpecification	Cat. No.	Storage
BroadDyeTM Terminator v3.5Ready Reaction Mix	220ml	35001	Store at -15 to -25℃
BroadDyeTM Terminator v3.5 5X Sequencing buffer	430ml	35002	Store at 4℃

 

Platforms:
  3730/3730xl DNA Analyzer 

  3500/3500xL Genetic Analyzer 

   3130/3130xl Genetic Analyzer 

  310 Genetic Analyzer 

Template Compatibility:
Plasmid DNA (≤15Kb)
Single Stranded DNA
Lambda DNA
Genomic DNA (Bacterial)
PCR Amplicons

Storage:  Store kit at -20°C. Avoid repeated freeze-thaw cycles.

 

Regulatory Statement:  for Research Use Only. Not for use in diagnostic procedures.