Summary:

 

Purpose

 

Our Quick Step Human Galectin-3 ELISA Kit is to assay Galectin-3 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

this ELISA kit uses Sandwich-ELISA as the method. the Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to Galectin-3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for Galectin-3 is added to each Microelisa stripplate well and incubated. Free components are washed away. the TMB substrate solution is added to each well. Only those wells that contain Galectin-3 and HRP conjugated Galectin-3 Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Galectin-3. You can calculate the concentration of Galectin-3 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

the standard curve of QS2065Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.